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active β catenin 8814 cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc active β catenin 8814 cell signaling technology
    Assessment of kidney AKI and fibrosis in L‐G6PC1‐low and L‐G6PC1‐high mice. L‐G6PC1‐low and L‐G6PC1‐high mice were generated from G6pc −/− mice and analyzed at 12 weeks of age. Age‐matched G6pc +/+ and G6pc +/− mice with a similar phenotype served as controls. (A) Western‐blot analyzes and quantitation of renal levels of E‐cadherin and N‐cadherin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (B) Western‐blot analyzes and quantitation of renal levels of Dkk3 and CTGF in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (C) Western‐blot analyzes and quantitation of renal levels of total <t>(β‐catenin‐T)</t> and active, dephosphorylated (β‐catenin‐A) β‐catenin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. For Western‐blot analyzes, densitometric quantification was performed and normalized against β‐Actin. Values represent the mean ± SEM. * p < 0.05, ** p < 0.005. (D) Immunohistochemical analysis of renal levels of active β‐catenin. Scale bar, 50 μm. (E) Quantitation of renal levels of nuclear localized active β‐catenin in control ( n = 3), L‐G6PC1‐low ( n = 3), and L‐G6PC1‐high ( n = 3) mice. Kidney sections were immunostained with HRP‐labeled anti‐active β‐catenin and the nuclei counterstained with hematoxylin. Images were digitized using the Motic EasyScan Infinity 60 scanner and analyzed with QuPath software (v0.4.3). Multiple annotations were selected across the entire renal cortex. The Nucleus DAB OD mean scoring method was used to identify moderate and strong optical density thresholds for nuclear‐stained β‐catenin.
    Active β Catenin 8814 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 810 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/active β catenin 8814 cell signaling technology/product/Cell Signaling Technology Inc
    Average 96 stars, based on 810 article reviews
    active β catenin 8814 cell signaling technology - by Bioz Stars, 2026-03
    96/100 stars

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    1) Product Images from "Liver‐Directed Gene Therapy Mitigates Early Nephropathy in Murine Glycogen Storage Disease Type Ia"

    Article Title: Liver‐Directed Gene Therapy Mitigates Early Nephropathy in Murine Glycogen Storage Disease Type Ia

    Journal: Journal of Inherited Metabolic Disease

    doi: 10.1002/jimd.70048

    Assessment of kidney AKI and fibrosis in L‐G6PC1‐low and L‐G6PC1‐high mice. L‐G6PC1‐low and L‐G6PC1‐high mice were generated from G6pc −/− mice and analyzed at 12 weeks of age. Age‐matched G6pc +/+ and G6pc +/− mice with a similar phenotype served as controls. (A) Western‐blot analyzes and quantitation of renal levels of E‐cadherin and N‐cadherin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (B) Western‐blot analyzes and quantitation of renal levels of Dkk3 and CTGF in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (C) Western‐blot analyzes and quantitation of renal levels of total (β‐catenin‐T) and active, dephosphorylated (β‐catenin‐A) β‐catenin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. For Western‐blot analyzes, densitometric quantification was performed and normalized against β‐Actin. Values represent the mean ± SEM. * p < 0.05, ** p < 0.005. (D) Immunohistochemical analysis of renal levels of active β‐catenin. Scale bar, 50 μm. (E) Quantitation of renal levels of nuclear localized active β‐catenin in control ( n = 3), L‐G6PC1‐low ( n = 3), and L‐G6PC1‐high ( n = 3) mice. Kidney sections were immunostained with HRP‐labeled anti‐active β‐catenin and the nuclei counterstained with hematoxylin. Images were digitized using the Motic EasyScan Infinity 60 scanner and analyzed with QuPath software (v0.4.3). Multiple annotations were selected across the entire renal cortex. The Nucleus DAB OD mean scoring method was used to identify moderate and strong optical density thresholds for nuclear‐stained β‐catenin.
    Figure Legend Snippet: Assessment of kidney AKI and fibrosis in L‐G6PC1‐low and L‐G6PC1‐high mice. L‐G6PC1‐low and L‐G6PC1‐high mice were generated from G6pc −/− mice and analyzed at 12 weeks of age. Age‐matched G6pc +/+ and G6pc +/− mice with a similar phenotype served as controls. (A) Western‐blot analyzes and quantitation of renal levels of E‐cadherin and N‐cadherin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (B) Western‐blot analyzes and quantitation of renal levels of Dkk3 and CTGF in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (C) Western‐blot analyzes and quantitation of renal levels of total (β‐catenin‐T) and active, dephosphorylated (β‐catenin‐A) β‐catenin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. For Western‐blot analyzes, densitometric quantification was performed and normalized against β‐Actin. Values represent the mean ± SEM. * p < 0.05, ** p < 0.005. (D) Immunohistochemical analysis of renal levels of active β‐catenin. Scale bar, 50 μm. (E) Quantitation of renal levels of nuclear localized active β‐catenin in control ( n = 3), L‐G6PC1‐low ( n = 3), and L‐G6PC1‐high ( n = 3) mice. Kidney sections were immunostained with HRP‐labeled anti‐active β‐catenin and the nuclei counterstained with hematoxylin. Images were digitized using the Motic EasyScan Infinity 60 scanner and analyzed with QuPath software (v0.4.3). Multiple annotations were selected across the entire renal cortex. The Nucleus DAB OD mean scoring method was used to identify moderate and strong optical density thresholds for nuclear‐stained β‐catenin.

    Techniques Used: Generated, Western Blot, Quantitation Assay, Control, Immunohistochemical staining, Labeling, Software, Staining



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    NEK2 activates the Wnt-signaling pathway in PRL-secreting pituitary tumor cells. (A) Immunoblot analysis of the key Wnt pathway-related proteins in GH3 cells transfected with NEK2 or empty vector. (B) RT-qPCR analysis of <t>Wnt/b-catenin</t> pathway downstream target genes in GH3 cells infected with lentivirus overexpressing a NEK2 or shRNA construct *P < 0.05, **P < 0.001, ***P < 0.0001. (C) TOP-Flash (TCF optimal promoter) assay used when NEK2 was overexpressed or knocked down in GH3 and MMQ cells.
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    Assessment of kidney AKI and fibrosis in L‐G6PC1‐low and L‐G6PC1‐high mice. L‐G6PC1‐low and L‐G6PC1‐high mice were generated from G6pc −/− mice and analyzed at 12 weeks of age. Age‐matched G6pc +/+ and G6pc +/− mice with a similar phenotype served as controls. (A) Western‐blot analyzes and quantitation of renal levels of E‐cadherin and N‐cadherin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (B) Western‐blot analyzes and quantitation of renal levels of Dkk3 and CTGF in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (C) Western‐blot analyzes and quantitation of renal levels of total (β‐catenin‐T) and active, dephosphorylated (β‐catenin‐A) β‐catenin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. For Western‐blot analyzes, densitometric quantification was performed and normalized against β‐Actin. Values represent the mean ± SEM. * p < 0.05, ** p < 0.005. (D) Immunohistochemical analysis of renal levels of active β‐catenin. Scale bar, 50 μm. (E) Quantitation of renal levels of nuclear localized active β‐catenin in control ( n = 3), L‐G6PC1‐low ( n = 3), and L‐G6PC1‐high ( n = 3) mice. Kidney sections were immunostained with HRP‐labeled anti‐active β‐catenin and the nuclei counterstained with hematoxylin. Images were digitized using the Motic EasyScan Infinity 60 scanner and analyzed with QuPath software (v0.4.3). Multiple annotations were selected across the entire renal cortex. The Nucleus DAB OD mean scoring method was used to identify moderate and strong optical density thresholds for nuclear‐stained β‐catenin.

    Journal: Journal of Inherited Metabolic Disease

    Article Title: Liver‐Directed Gene Therapy Mitigates Early Nephropathy in Murine Glycogen Storage Disease Type Ia

    doi: 10.1002/jimd.70048

    Figure Lengend Snippet: Assessment of kidney AKI and fibrosis in L‐G6PC1‐low and L‐G6PC1‐high mice. L‐G6PC1‐low and L‐G6PC1‐high mice were generated from G6pc −/− mice and analyzed at 12 weeks of age. Age‐matched G6pc +/+ and G6pc +/− mice with a similar phenotype served as controls. (A) Western‐blot analyzes and quantitation of renal levels of E‐cadherin and N‐cadherin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (B) Western‐blot analyzes and quantitation of renal levels of Dkk3 and CTGF in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (C) Western‐blot analyzes and quantitation of renal levels of total (β‐catenin‐T) and active, dephosphorylated (β‐catenin‐A) β‐catenin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. For Western‐blot analyzes, densitometric quantification was performed and normalized against β‐Actin. Values represent the mean ± SEM. * p < 0.05, ** p < 0.005. (D) Immunohistochemical analysis of renal levels of active β‐catenin. Scale bar, 50 μm. (E) Quantitation of renal levels of nuclear localized active β‐catenin in control ( n = 3), L‐G6PC1‐low ( n = 3), and L‐G6PC1‐high ( n = 3) mice. Kidney sections were immunostained with HRP‐labeled anti‐active β‐catenin and the nuclei counterstained with hematoxylin. Images were digitized using the Motic EasyScan Infinity 60 scanner and analyzed with QuPath software (v0.4.3). Multiple annotations were selected across the entire renal cortex. The Nucleus DAB OD mean scoring method was used to identify moderate and strong optical density thresholds for nuclear‐stained β‐catenin.

    Article Snippet: Nuclear‐translocated active β‐catenin was analyzed by immunohistochemistry on paraffin‐embedded mouse kidney sections using a rabbit monoclonal antibody specific for non‐phosphorylated (active) β‐catenin (#8814, Cell Signaling Technology).

    Techniques: Generated, Western Blot, Quantitation Assay, Control, Immunohistochemical staining, Labeling, Software, Staining

    NEK2 activates the Wnt-signaling pathway in PRL-secreting pituitary tumor cells. (A) Immunoblot analysis of the key Wnt pathway-related proteins in GH3 cells transfected with NEK2 or empty vector. (B) RT-qPCR analysis of Wnt/b-catenin pathway downstream target genes in GH3 cells infected with lentivirus overexpressing a NEK2 or shRNA construct *P < 0.05, **P < 0.001, ***P < 0.0001. (C) TOP-Flash (TCF optimal promoter) assay used when NEK2 was overexpressed or knocked down in GH3 and MMQ cells.

    Journal: Journal of Cancer

    Article Title: NEK2 regulates cellular proliferation and cabergoline sensitivity in pituitary adenomas

    doi: 10.7150/jca.52937

    Figure Lengend Snippet: NEK2 activates the Wnt-signaling pathway in PRL-secreting pituitary tumor cells. (A) Immunoblot analysis of the key Wnt pathway-related proteins in GH3 cells transfected with NEK2 or empty vector. (B) RT-qPCR analysis of Wnt/b-catenin pathway downstream target genes in GH3 cells infected with lentivirus overexpressing a NEK2 or shRNA construct *P < 0.05, **P < 0.001, ***P < 0.0001. (C) TOP-Flash (TCF optimal promoter) assay used when NEK2 was overexpressed or knocked down in GH3 and MMQ cells.

    Article Snippet: The primary antibodies we used were as follows: non-phospho (active) β-catenin (8814, Cell Signaling Technology [CST]), caspase-3 (9662, CST), cleaved caspase-3 (9664, CST), USP7 (4833, CST), NEK2 (ab115731, abcam), cyclin D1 (55506, CST), c-myc (18583, CST), phosphor-erk1/2 (4370, CST), Erk1/2 (4695, CST), GAPDH (2118, CST), α-tubulin (2125, CST), GFP (2956, CST), and ubiquitin (3936, CST).

    Techniques: Western Blot, Transfection, Plasmid Preparation, Quantitative RT-PCR, Infection, shRNA, Construct, Promoter Assay